On 19 and 20 March 2019 hosting partner Instituto Nacional de Saúde welcomed the General Assembly to Maputo, Mozambique, for the second PanACEA II Annual Meeting. Highlights of the second year of the PanACEA II programme were the completion of the HR1 trial, start of the postgraduate programme, site assessment visits and capacity development activities (e.g. MBLA implementation) and the SimpliciTB collaboration. For the third year, the WP2 trials and SimpliciTB kick-off within PanACEA are planned. During the meeting each site presented ongoing scientific studies to exchange knowledge and experience.